General overview of the lrn and recommendations the primary function of the laboratory response network lrn for biological threat preparedness and response is to provide rapid detection of biothreat and emerging agents. Material safety data sheet section i product information product name or identity. Evaluation of ureamotilityindole medium for recognition. Kligler iron agar productinformation product number k2260 product description kligler iron agar is recommended as a differential medium in the study of the gramnegative intestinal microorganisms, on the basis of their fermentative ability for dextrose and lactose. Reactions of kia helps to includeexclude particular bacterial isolate in the family enterobacteriaceae.
Kligler iron agar dehydrated culture media for microbiology. History in 1911, russell described a medium containing two sugars for the isolation of typhoid bacilli in urine. Stewart 1 nature volume 183, pages 1537 1538 1959 cite this article. Triple sugariron agar medium mm021 triple sugar iron agar medium is recommended for identification of gramnegative enteric bacilli on the basis of dextrose, lactose and sucrose fermentation and hydrogen sulphide production in accordance with indian pharmacopoeia. Themethodsare basedonprimarydifferentiation of the various groups of bacteria by the use of kligler iron agar and lysine iron agar. Pdf rapid biochemical screening for salmonella, shigella. This page is the site outline for bacteriologicallyrelevant items at. Kligler iron agar is recommended for differentiation of enteric gram negative bacilli from clinical specimens and food samples. Oxidase testing from kliglers iron agar and triple sugar iron agar. Although both combinations enabled the presumptive recognition and differentiation of salmonella and shigella species, an analysis of data indicated that the combination of kligler iron agar and ureamotilityindole medium performed better than the combination of kligler iron agar and lysine iron agar in detecting salmonella and shigella species. The salmonella screen consisted of kligler iron agar kia, motilityindoleurea tryptophandeamination semisolid medium miutda, and the. Kligler iron agar, in addition to peptone, hm peptone b and yeast extract, contains lactose and glucose dextrose, which enables the differentiation of species of enteric bacilli.
Page 1 of 7 sds 7140 rev 0 march 2016 section 1 identification of the substancemixture and of the company 1. Bromcresol purple acts as the color indicator in the. Product summary and explanation kligler iron agar is a modification of kligler s original formula, recommended to identify pure cultures of. The basal medium of kia is composed of casein and meat peptones with the addition of lactose and dextrose. Kligler iron agar kia intended use remel kligler iron agar kia is a solid medium recommended for use in qualitative procedures for differentiation of enteric gramnegative bacilli on the basis of dextrose and lactose fermentation and hydrogen sulfide. Hajna developed the formulation for tsi agar by adding sucrose to the double sugar dextrose and lactose formulation of kligler iron agar. Kligler iron agar kia or the identificaiton of enteric. Principles of the procedure kligler iron agar, in addition to casein and meat peptones, contains lactose and dextrose which enable the differentiation. His studies at that time dealt with classification of bacteria and their biochemical properties.
Heat stirring constantly to boiling point and boil for 1 minute. Not only does this medium perform most of the functions of kligler iron agar but, in addition, its sucrose content permits the recognition and exclusion of sucrosefermenting species. Oxidase tests performed from the surface of kliglers iron agar and triple sugar iron. In the kliglers iron agar kia test if an organism can only. Since there is only a small amount of sugar dextrose in the medium, acid production due to its fermentation is very limited and. Agar is polysaccharide extract obtained from seaweed. He developed the kligler iron agar medium for the isolation and identification of intestinal bacteria, which is still in. Aprocedure is described for identifying members ofthe family enterobacteriaceaeisolatedfromclinical specimens. Kligler iron agar kia combines features of kliglers lead acetate medium and russells double sugar agar. Kligler iron agar is a modification of kligler s original formula, combining features of the kligler s lead acetate medium with the features of russells double sugar agar. The growth characteristics of enterobacteriaceae on tsi media are slightly different from those on kligler iron agar.
Biochemical differentiation of the enterobacteriaceae. Quantitative data on the toxicity of this product is not available. These organisms may ferment lactose slowly or not at all during the incubation period, but they attack sucrose readily. Kligler agar msds material safety data sheet or sds, coa and coq, dossiers, brochures and other available documents. Kligler iron agar kia may be replaced by triple sugar iron agar tsi but only for enteric pathogens. Kligler iron agar is a combination of the lead acetate medium described by kligler 9 and. Kligler began publishing research papers at the age of 24, immediately after his graduation. Biochemical of the enterobacteriaceae with the aid of. The phenylalanine test on kligler s iron agar for the identification of proteus d. Cm0033 risk phrases safety phrases ec classification this preparation is not classified as dangerous according to the ec directive 88379eec.
Enterobacteriaceae with the aid of lysine iron agar. Section 1 identification of the substancemixture and of. Kligler iron agar 7140 intended use kligler iron agar is used for the differentiation of microorganisms on the basis of dextrose and lactose fermentation and hydrogen sulfide production. Phenol red is the ph indicator, which exhibits a colour change in response to acid produced during the fermentation of sugars. Basic laboratory procedures in clinical bacteriology. Pancreatic digest of gelatin and yeast extract provide nitrogen, vitamins, amino acids, and other essential factors required for bacterial growth. Kliglers iron agar test procedure, uses and interpretation. Principle, procedure and results kligler s iron agar kia is used for the detection of.
The methods are based on primary differentiation of the various groups of bacteria by the use of kligler iron agar and lysine iron agar. Packaging code content packaging 30087 10 tubes x 10 ml 10 tubes in cardboard box ph of the medium 7. Page 25 material safety data sheet lewis university. Tsi agar medium was developed based on kliglers iron agar, which had been used for the determination of lactosefermentative bacteria, by addition of sucrose. Media are of different types on consistency and chemical composition. Kligler iron agar dehydrated culture media for microbiology preparation suspend 52 grams of the medium in one litre of distilled water. The phenylalanine test on kliglers iron agar for the. Kligler iron agar kligler hajna is a solid culture medium for the identification of enterobacteriaceae, based on the fermentation of dextrose and lactose and the production of hydrogen sulfide. Dehydrated culture media of pinkish beige colour preparation. Kligler iron agar intended use kligler iron agar is used for the identification of enterobacteria by the rapid detection of lactose and glucose fermentation with or without gas production, as well as the production of hydrogen sulfide.
Kligler added lead acetate to russell double sugar agar, resulting in a medium capable of differentiating typhoid, paratyphoid, and dysentery. These triple sugar iron tsi agar slants contain sucrose, lactose, glucose and iron ferrous sulfate. Make a suspension of 52 g in 1 l of distilled water. The current formulation of kligler iron agar combines features of kligler s lead acetate medium with those of russells double sugar agar. If an organism cannot ferment glucose, then in kia, alkaline slantalkalinebutt no change reaction is observed, this reaction alone is sufficient to exclude an isolate from the enterobacteriaceae family. Biochemical differentiation of the enterobacteriaceae with the aid of lysine iron agar. Kligler iron agar k11100 safety data sheet according to 19072006ec reach, 4532010eu, 2015830eu section 4. Get a printable copy pdf file of the complete article 765k, or click on a. Kligler iron agar 7140 7140a kligler iron agar is used for the differentiation of microorganisms on the basis of dextrose and lactose fermentation and hydrogen sulfide production in a laboratory setting. Solid media is used for the isolation of bacteria as pure culture.
Mix well and dissolve by heating with frequent agitation. Sugar fermentation is shown by acid production, which turns the indicator from red to yellow. Kligler iron agar safety data sheet date of issue 11022009. Kligler iron agar dehydrated culture media for microbiology physical data. Kligler was one of the pioneers of modern medical research in mandatory palestine, studying as varied a field as bacteriology, parasitology, virology, nutrition, epidemiology and public health. He used his knowledge of their growth needs in order to develop a new medium kligler iron agar 7 for which he gained an. Acute and delayed effects are indicated in sections 2 and 11. Biochemical differentiation of the enterobacteriaceae with. Kligler s iron agar kia is used for the detection of carbohydrate fermentation.
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